Diverse Growth Kinetics in Shaken Suspension Culture of the Model Eukaryote Dictyostelium discoideum and Confirmation of Lagless Growth
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چکیده
منابع مشابه
Contact-mediated cell-assisted cell proliferation in a model eukaryotic single-cell organism: an explanation for the lag phase in shaken cell culture.
In cell culture, when cells are inoculated into fresh media, there can be a period of slow (or lag phase) growth followed by a transition to exponential growth. This period of slow growth is usually attributed to the cells' adaptation to a new environment. However, we argue that, based on observations of shaken suspension culture of Dictyostelium discoideum, a model single-cell eukaryote, this ...
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In Dictyostelium discoideum, 16 mutants in which cells differentiate into spores and stalk cells without normal morphogenesis were isolated. All these mutants are rapidly developing and capable of differentiating in a shaken suspension of phosphate buffer. The developmental kinetics of specific activities of enzymes in one of the mutants, HTY 1851, cultured in the suspension was compared with t...
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These kinetic mechanisms of eukaryote cells (type Dictyostelium discoideum) were tested in a two-plate cell detachment assay with the coated substrates as test plates and stainless steel discs as counter plates. The suspension of the cells in Sörensen buffer rested between the two plates, retained by the balance between gravity and capillary forces. All coatings influenced the cells leading to ...
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